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Become a Certified DOT Specimen Collector for $320.

Master the essentials of DOT drug test collections with comprehensive, self-paced training covering 49 CFR Part 40, collection procedures, chain of custody, site security, mock collections and the knowledge needed to build your skills as a DOT specimen collector.

49 CFR Part 40Training focused on DOT collection requirements
Self-PacedWork through the training on your schedule
Mock CollectionsComplete five online video mock collections
CertificateCertificate after five error-free mock collections
DOT Collector Training

Build the knowledge and skills to perform DOT specimen collections.

The screenings4u training program covers the core requirements involved in DOT urine drug test collections, from collector responsibilities and collection-site security to Custody & Control Form completion and specimen handling.

  • 49 CFR Part 40 fundamentals
  • DOT collector requirements and responsibilities
  • Collection site security and required supplies
  • Custody & Control Form completion
  • Urine specimen collection procedures
  • Monitored and direct observation collections

Training built around the collection process.

Work through the curriculum, complete the included mock collections and build familiarity with the procedures and documentation involved in DOT specimen collection.

3 HoursDOT Specimen Collector Training
5 MocksDOT online video mock collections totaling about 2 hours
DownloadsTraining materials, guides and forms
CertificateCompletion certificate after five error-free mock collections
Course Curriculum

Ten units covering the fundamentals of DOT specimen collection.

The curriculum follows the major areas identified by screenings4u for becoming familiar with DOT urine specimen collection procedures and related responsibilities.

01

Introduction & Background

DOT regulations, regulated agencies, safety-sensitive positions, reasons for testing, drug testing panels, acceptable testing methods and approved laboratories.

02

The Collector

Who may become a collector, collector training requirements, collector and employer responsibilities, and restrictions placed on collectors.

03

Collection Site & Supplies

Collection-site requirements, donor privacy, site security, required collection supplies and collection-kit contents.

04

Custody & Control Form

DOT release, waiver and privacy forms, the CCF, required information, completion steps and proper distribution.

05

The Collection Process

Testing considerations, collection steps, donor instructions, specimen inspection, splitting and preparing specimens for laboratory shipment.

06

Monitored & Direct Observation

Monitored collections, who may perform them, required procedures and documentation, plus direct observation requirements.

07

Shy Bladder

Shy bladder procedures including starting without delay, proper donor instructions, required steps and documentation.

08

Problem Specimens

How to respond to temperature-out-of-range, adulterated and substituted specimens and properly document the collection.

09

Unusual Collections & Mishaps

Refusals, missing signatures or initials, catheter situations, fatal and correctable flaws, torn seals and Public Interest Exclusion topics.

10

Non-Regulated Testing

Key differences between DOT and non-DOT collections and conducting DOT and non-DOT collections on the same donor.

11

Starter Guide

Access to a DOT Specimen Collector Starter Guide and supporting reference materials.

12

Policy Guides

Reference materials include sample DOT and NON-DOT Drug & Alcohol Policy guides.

What's Included

More than a training video.

The training package combines instruction, mock collections, downloadable resources and a certificate path.

Training & Practice

Build familiarity with the collection process through structured instruction and mock collection exercises.

  • 3 hours of DOT Specimen Collector Training
  • 5 DOT online video mock collections
  • Approximately 2 hours of mock collection content
  • Self-paced learning modules
  • 49 CFR Part 40-focused curriculum

Resources & Certification

Complete the required mock collections and receive access to supporting materials for your training.

  • Downloadable training materials and forms
  • DOT Specimen Collector Starter Guide
  • Sample DOT Drug & Alcohol Policy
  • Sample NON-DOT Drug & Alcohol Policy
  • Completion certificate after 5 error-free mock collections
How Training Works

Learn the process. Practice the process. Complete the training.

Move through the self-paced curriculum and mock collections in a structured sequence designed around the major components of DOT specimen collection.

01

Complete the Training Modules

Work through the curriculum covering DOT regulations, collector requirements, site security, documentation and collection procedures.

02

Review Collection Procedures

Build familiarity with donor instructions, specimen handling, Custody & Control Form completion and unusual collection situations.

03

Complete Five Mock Collections

Work through the five online video mock collections included with the training.

04

Earn Your Completion Certificate

Receive a completion certificate after completing five error-free mock collections.

Training Offerings & Pricing

Choose the training path that fits your goals.

screenings4u has four training options, each with 60-day access and a single-person enrollment.

$320

DOT Specimen Collector Training

1 person, 60-day access, self-paced learning modules, DOT mock collections, collection-site security, collector requirements, regulated agencies, collector certificate, specimen collection process and CCF completion.

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$360

DOT Specimen Collector Training + Hair

Includes the DOT Specimen Collector Training package plus Hair Drug Test Training and a Hair Drug Test Certificate.

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$550

DOT Specimen Collector Train the Trainer

Includes the collector training curriculum plus instructor materials, the ability to train others and an Instructor Certificate.

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$600

DOT Collector Train the Trainer + Hair

Includes the Train the Trainer package plus Hair Drug Test Training and a Hair Drug Test Certificate.

Enroll Now →
Training FAQ

Questions about DOT Specimen Collector Training?

Review the core training options and included features before enrolling.

The current offering includes 1 person, 60-day access, self-paced learning modules, 49 CFR Part 40-focused training, DOT mock collections, collection-site security, collector requirements, DOT regulated agencies, a DOT collector certificate, specimen collection process training and chain-of-custody completion.
The Hair option includes the DOT Specimen Collector Training package plus Hair Drug Test Training and a Hair Drug Test Certificate.
The Train the Trainer option includes the collector training curriculum plus instructor materials, the ability to train others and an Instructor Certificate.
The training page states that a completion certificate is received after completing five error-free mock collections.

Ready to become a DOT specimen collector?

Explore the training options and start building your collection skills.